烟曲霉过敏原Asp f 1检测试剂盒
Asp f 1 ELISA kit
- EL-AF1
- INDOOR BIOTECHNOLOGIES
- 上海市
- 一个月内
- 1盒
- 0 元
- 2022-05-24 16:42:11
上海博升生物科技有限公司
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- 英文名称
- Asp f 1 ELISA kit
| ELISA kit for measuring Aspergillus fumigatus allergen, Asp f 1. |
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| Sensitivity: | 0.08ng/ml |
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| Limit of Detection: | 0.32ng/ml |
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| Assay Performance: | Inquire |
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| Contents: |
Monoclonal antibody 4A6, Asp f 1 Standard, Rabbit anti-Asp f 1; sufficient reagents for 10 microtiter plates. |
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| Storage: | Store at 4°C. |
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| Not Provided: |
50mM carbonate/bicarbonate buffer, pH 9.6 |
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| Notes: | ||||
| Product Resources: | EL-AF1 Certificate of Analysis |
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| For research and commercial use in vitro: not for human in vivo or therapeutic use. |
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| Certificate of Analysis |
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| Monoclonal Antibody: | 4A6 |
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| Vial: | Red Cap; 100µl |
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| Immunogen: | Asp f 1 |
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| Isotype: | Mouse IgG1 |
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| Specificity: | Binds to a common epitope on Aspergillus fumigatus allergen, Asp f 1. |
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| Purification: | Produced in tissue culture and purified by chromatography using Protein G. Single heavy and light chain bands on SDS-PAGE. |
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| Concentration: | 2mg/ml in phosphate buffered saline, pH 7.4. Based on A280 for IgG (1.42=1mg/ml), 0.22μm filtered, preservative free. |
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| Certificate of Analysis |
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| Antibody: | Polyclonal antibody raised against partially purified Asp f 1 (1,2). |
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| Vial: | Brown Cap; 100µl |
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| Specificity: | The pAb contains IgG antibodies to Asp f 1 as well as IgG antibodies to other Aspergillus allergens. |
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| Activity: | The pAb is in phosphate buffered saline containing 1%BSA 50%glycerol PBS, pH 7.4. The pAb has been 0.22µm filtered and should be diluted 1/1000 for use in Asp f 1 ELISA. |
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| Certificate of Analysis |
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| Allergen Standard: | rAsp f 1 |
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| Vial: | White Cap; 400µl |
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| Composition: | Recombinant Asp f 1 prepared in 1% BSA/50% glycerol/PBS, 0.22μm filtered, preservative free, pH 7.4 |
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| Concentration: | 400ng/ml rAsp f 1 |
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Concentration: |
The Asp f 1 concentration was determined by amino acid analysis. Asp f 1 and Asp r 1 (mitogillin) are 95% homologous (differing by 4 amino acid residues) and are antigenically and functionally indistinguishable (1). | |||
| References: |
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| 1. Arruda, LK, Platts-Mills, TAE, Fox, JW and Chapman, MD (1990). Aspergillus fumigatus allergen I, a major IgE binding protein, is a member of the mitogillin family of cytotoxins. J. Exp. Med., 172:1529-1532. 2. Arruda LK, Platts-Mills TAE, Longbottom JL, Chapman MD (1992). Aspergillus fumigatus: Identification of 16kd, 18kd and 45kd antigens recognized by human IgG and IgE antibodies and murine monoclonal antibodies. J. Allergy Clin. Immunol. 89:1166-1176. 3. Arruda LK, Mann BJ, Chapman MD (1992). Selective expression of a major allergen and cytotoxin, Asp f 1, in Aspergillus fumigatus. J. Immunol. 149:3354- 3359. 4. Sporik RB, Arruda LK, Woodfolk J, Chapman MD, Platts-Mills TAE (1993). Environmental exposure to Aspergillus fumigatus allergen 1 (Asp f 1). Clin. Exp. Allergy 23:326-331. 5. El-Dahr JM, Fink R, Selden R, Arruda LK, Platts-Mills TAE, Heymann PW (1994). Development of immune responses to Aspergillus at an early age in children with cystic fibrosis. Am. J. Respir. Crit. Care Med. 150:1513-1518. 6. Vailes LD, Sridhara S, Cromwell O, Weber B, Breitenbach M, Chapman MD (2001). Quantitation of the major fungal allergens, Alt a 1 and Asp f 1, in commercial allergenic products. J. Allergy Clin. Immunol. 107:641-646. 7. Chapman MD (2006). Challenges associated with indoor moulds: Health effects, immune response and exposure assessment. Medical Mycology. 44:S29-S32. |
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| (For complete reference list see EL-AF1 Certificate of Analysis) | ||||
