花生过敏原Ara h1检测试剂盒
- EL-AH1
- INDOOR BIOTECHNOLOGIES
- 上海市
- 一个月内
- 1盒
- 0 元
- 2022-05-24 16:10:00
上海博升生物科技有限公司
- 英文名称
- Ara h 1 ELISA kit
| ELISA kit for measuring Arachis hypogaea allergen, Ara h 1. |
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| Antibodies: | 2C12/2F7 |
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| Sensitivity: | 4ng/ml |
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| Limit of Detection: | 31.5ng/ml |
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| Assay Performance: | Intra-assay CV% of 3.1; Inter-assay CV% of 11.8 |
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| Contents: |
Monoclonal antibody 2C12, Ara h 1 Allergen Standard, Biotinylated |
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| Storage: | Store antibodies at 4°C; store ST-AH1 at -20°C upon arrival (standard has 3 month shelf life). |
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| Not Provided: |
50mM carbonate/bicarbonate buffer, pH 9.6 |
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| Notes: |
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| Product Resources: | EL-AH1 Certificate of Analysis |
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| For research and commercial use in vitro: not for human in vivo or therapeutic use. |
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| Certificate of Analysis |
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| Monoclonal Antibody: | 2C12 (clone 2C12 A11 A3) |
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| Vial: | Red Cap; 100µl |
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| Immunogen: | Ara h 1 |
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| Isotype: | Mouse IgG1 |
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| Specificity: | Binds to species specific epitope present on Arachis hypogaea allergen, Ara h 1. |
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| Purification: | Produced in vitro by BioVectra dcl bioreactors and purified by chromatography using Protein G. Single heavy and light chain bands on SDS-PAGE. |
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| Concentration: | 2.7 mg/ml in phosphate buffered saline, pH 7.4. Based on A280 for IgG (1.42=1mg/ml), 0.22μm filtered, preservative free. |
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| Certificate of Analysis |
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| Monoclonal Antibody: | 2F7 (clone 2F7 C12 D10) |
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| Vial: | Brown Cap; 100µl |
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| Immunogen: | Ara h 1 |
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| Isotype: | Mouse IgG1 |
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| Specificity: | Binds to species specific epitope present on Arachis hypogaea allergen, Ara h 1. |
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| Purification: | Produced in ascites and purified by affinity chromatography using Protein G. Single heavy and light chain bands on SDS-PAGE. |
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| Biotinylation: | Biotinylated and titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22µm filtered, preservative free. |
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| Certificate of Analysis |
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| Allergen Standard: | nAra h 1 |
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| Vial: | White Cap; 400µl |
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| Composition: | Naturally purified Ara h 1 prepared in 1% BSA/50% glycerol/PBS, pH 7.4. |
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| Concentration: | 20,000ng/ml Ara h1 |
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| Calibration: | The Ara h 1 concentration of the purified Ara h 1 was determined by OD280. |
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| Stability/Storage: | Please store the standard at -20ºC (+/- 5°C). |
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References: 1. Pomés A, Helm RM, Bannon GA, Burks AW, Tsay A, Chapman MD. Monitoring peanut allergen in food products by measuring Ara h 1. J Allergy Clin Immunol 2003;111:640-5. 2. Pomés A, Vinton R, Chapman MD. Peanut allergen (Ara h 1) detection in foods containing chocolate. J Food Prot. 2004 Apr:67 (4):793-8. 3. Perry TT, Conover-Walker MK, Pomés A, Chapman MD, Wood RA. Distribution of peanut allergen in the environment. J Allergy Clin Immunol. 2004 May;113(5):973-6. 4. Maloney JM, Chapman MD, Sicherer SH. Peanut allergen exposure through saliva: assessment and interventions to reduce exposure. J Allergy Clin Immunol. 2006 Sep;118(3):719-24. |
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| (For complete reference list see EL-AH1 Certificate of Analysis) | ||||
