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豚鼠尿蛋白过敏原Guinea Pig Urinary Protein检测试剂盒
Guinea Pig Urinary Protein ELISA kit
  • EL-GPUP
  • INDOOR BIOTECHNOLOGIES
  • 上海市
  • 一个月内
  • 1盒
  • 0
  • 2022-05-24 15:37:54

上海博升生物科技有限公司

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  • 英文名称
  • Guinea Pig Urinary Protein ELISA kit
ELISA kit for measuring Guinea Pig Urinary Protein allergen, GPUP.

Antibodies: pAb/pAb

Sensitivity: 0.5ng/ml

Limit of Detection: 7.8ng/ml

Assay Performance: Inquire

Contents:

Rabbit polyclonal antiserum, nGPUP Standard, Rabbit polyclonal antiserum; sufficient reagents for 10 microtiter plates.

Storage: Store at 4°C

Not Provided:

50mM carbonate/bicarbonate buffer, pH 9.6

Phosphate buffered saline, pH 7.4, containing 0.05% Tween 20 (PBS-T)

Bovine serum albumin

Peroxidase conjugated Goat anti. Rabbit IgG

1mM ABTS in 70mM citrate-phosphate buffer, pH 4.2

Notes:

 
Product Resources: EL-GPUP Certificate of Analysis

For research and commercial use in vitro: not for human in vivo or therapeutic use.

  Certificate of Analysis

Monoclonal Antibody: Rabbit polyclonal antiserum

Immunogen: Guinea Pig Urinary Protein

Isotype: Multiple

Specificity: Binds to guinea pig urinary proteins. Reacts with guinea pig epithelial and hair extracts. Does not recognize rat or mouse urinary proteins; does not react with rabbit or hamster epithelial and hair extracts.

Purification: Affinity chromatography using recombinant Protein G.

Concentration: 1-2mg/ml in phosphate buffered saline, pH 7.4 Based on A280 for IgG (1.42=1mg/ml), 0.22μm filtered, preservative free.


  Certificate of Analysis

Antibody: Rabbit polyclonal antiserum

Specificity:

Binds to guinea pig urinary proteins. Reacts with guinea pig epithelial and hair extracts. Does not recognize rat or mouse urinary proteins; does not react with rabbit or hamster epithelial and hair extracts.
Biotinylation:

Biotinylated and titrated for use in ELISA at 1/1,000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4. 0.22μm filtered, preservative free.

  Certificate of Analysis

Allergen Standard: nGPUP

Composition: Purified natural guinea pig urinary protein prepared in

1% BSA/50% glycerol/PBS, pH 7.4.

Concentration:

10,000ng/mL as determined by Advanced Protein Assay

References:

 
1. Hilger C, Swiontek K, Kler S, Diederich C, Lehners C, Vogel L, Vieths S, Hentges F. Evaluation of two new recombinant guinea-pig lipocalins, Cav p 2 and Cav p 3, in the diagnosis of guinea-pig allergy. Clin Exp
Allergy. 2011 Jun; 41(6):899-908.
 

2. Ferraz E, Arruda LK, Bagatin E, Martinez EZ, Cetlin AA, Simoneti CS, Freitas AS, Martinez JA, Borges MC, Vianna EO. Laboratory animals and respiratory allergies: the prevalence of allergies among laboratory animal workers and the need for prophylaxis. Clinics (Sao Paulo). 2013 Jun; 68(6): 750–759. 

(For complete reference list see EL-GPUP Certificate of Analysis)

 

 

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