德国小蠊过敏原Bla g1检测试剂盒
Bla g 1 ELISA kit (EL-BG1)
- EL-BG1
- INDOOR BIOTECHNOLOGIES
- 上海市
- 一个月内
- 1盒
- 0 元
- 2022-05-24 15:32:23
上海博升生物科技有限公司
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- 英文名称
- Bla g 1 ELISA kit (EL-BG1)
| ELISA kit for measuring Blattella germanica allergen, Bla g 1. |
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| Antibodies: | 10A6/pAb |
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| Sensitivity: | 0.20ng/ml |
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| Limit of Detection: | 0.80ng/ml |
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| Assay Performance: | Inquire |
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| Contents: |
Monoclonal antibody 10A6, Bla g 1 Standard, Rabbit anti-Bla g 1; sufficient reagents for 10 microtiter plates. |
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| Storage: | Store at 4°C |
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| Not Provided: |
50mM carbonate/bicarbonate buffer, pH 9.6 |
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| Notes: | ||||
| Product Resources: | EL-BG1 Certificate of Analysis |
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| For research and commercial use in vitro: not for human in vivo or therapeutic use. |
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| Certificate of Analysis |
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| Monoclonal Antibody: | 10A6 (clone 10A6 F5 E1) |
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| Immunogen: | Bla g 1 |
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| Isotype: | Mouse IgG1 |
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| Specificity: | Binds to a common epitope on cockroach Blattella germanica allergen Bla g 1 and Periplaneta americana allergen, Per a 1. |
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| Purification: | From ascites by ammonium sulphate precipitation and by HPLC using recombinant protein G. Single heavy and light chain bands on SDS-PAGE. |
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| Concentration: | 1mg/ml in phosphate buffered saline, pH 7.4 Based on A280 for IgG (1.42=1mg/ml), 0.22μm filtered, preservative free. |
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| Certificate of Analysis |
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| Antibody: | Polyclonal antibody raised against recombinant Bla g 1. |
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| Specificity: | The pAb contains IgG antibodies to Bla g 1 and does not react with Bla g 2. |
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| Activity: | The pAb is in phosphate buffered saline, pH 7.4, containing 1%BSA/50% glycerol. The pAb has been 0.22μm filtered and should be diluted 1/1000 for use in Bla g 1 ELISA. |
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| Certificate of Analysis |
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| Allergen Standard: | rBla g 1 |
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| Composition: | Purified from Pichia pastoris culture by affinity chromatography and prepared in 1% BSA, 50% glycerol/PBS, pH 7.4. |
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Concentration: |
1000ng/ml |
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| Calibration: |
The concentration of the purified recombinant Bla g 1 was determined by amino acid analysis. There are no national or international reference standards for cockroach allergens. |
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| Notes: |
Bla g 1 measurements were previously reported in arbitrary units (U/ml). Studies carried out with rBla g 1, Lot 36019 have determined that 1 Unit is ~100ng Bla g 1 protein. |
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| References: |
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| 1. Pollart SM, Smith TF, Morris E, Gelber LE, Platts-Mills TAE, Chapman MD. Environmental exposure to cockroach allergens: analysis with monoclonal Antibody based enzyme immunoassays. J Allergy Clin Immunol 87:505-510, 1991. | ||||
| 2. Gelber LE, Seltzer LH, Bouzoukis JK, Pollart SM, Chapman MD, Platts-Mills TAE. Sensitization ad exposure to indoor allergens as risk factors for asthma among patients presenting to hospital. Amer Rev Respir Dis 147:573-578, 1993. | ||||
| 3. Rosenstreich DL, Eggleston P, Kattan M, Baker D, Slavin RG, Gergen P, Mitchell H, McNiff-Mortimer K, Lynn H, Ownby D, Malveaux F. The role of cockroach allergy and exposure to cockroach allergen in causing morbidity among inner-city children with asthma. New Eng J Med 336:1356-1363, 1997. | ||||
| 4. Arruda LK, Vailes LD, Ferriani VP, Santos AB, Pomés A, Chapman MD. Cockroach allergens and asthma. J Allergy Clin Immunol 107:419-428, 2001. | ||||
| 5. Pomés A, Vailes LD, Helm RM, Chapman MD. IgE reactivity of tandem repeats derived from cockroach allergen, Bla g 1. Eur J Biochem 269:3086-3092, 2002. | ||||
| 6. Nalyanya G, Gore JC, Linker HM, Schal C. German cockroach allergen levels in North Carolina schools: comparison of integrated pest management and conventional cockroach control. J Med Entomol 46:420-427, 2009. | ||||
| (For complete reference list see EL-BG1 Certificate of Analysis) | ||||
