CRISPR Cas12a-RPA Lyophilized RNA Detection Kit (Two-Step)
- C12ALRT-96 (for 96T)
- sbs赛百盛
- 北京市
- 一周内
- 按需
- 议价
- 2025-03-03 12:51:51
北京赛百盛基因技术有限公司
- 英文名称
- CRISPR Cas12a-RPA Lyophilized RNA Detection Kit (Two-Step)
- CAS号
- C12ALRT-96 (for 96T)
Cas12a forms a functional complex with crRNA and "slides" along the target nucleic acid sequence. Upon successful pairing, Cas12a is specifically activated and trans-cleaves surrounding reporter molecules. This kit combines isothermal amplification technology with Cas12a, featuring high sensitivity, high specificity, and a high signal-to-noise ratio. It is widely used in molecular diagnostics, enabling rapid and accurate detection of pathogens.
Cat. No.: C12ALRT-96 (for 96T)
Cat. No.: C12ALRT-480 (for 480T)
Description
Cas12a forms a functional complex with crRNA and "slides" along the target nucleic acid sequence. Upon successful pairing, Cas12a is specifically activated and trans-cleaves surrounding reporter molecules. This kit combines isothermal amplification technology with Cas12a, featuring high sensitivity, high specificity, and a high signal-to-noise ratio. It is widely used in molecular diagnostics, enabling rapid and accurate detection of pathogens.
Kit Components
No. 1 |
Item Reaction Buffer (2X) |
Size 1000 μL |
2 |
Core Mix (4X) |
500 μL |
3 |
Positive Control (10X) (includes primer and DNA template) |
30 μL |
4 |
Cleavage Buffer (10X) |
240 μL |
5 |
Cas12a Protein (10 μM) |
20 μL |
6 |
crRNA for Positive Control (20X) |
20 μL |
7 |
Reporter (4 μM) |
80 μL |
8 |
Starter (10X) |
200 μL |
Additional Required Materials
- Fluorometer for reading FAM signals (e.g., qPCR instrument)
- Pipettes
- Nuclease-free water
- Target sequence-specific primers for isothermal amplification
- crRNA/gRNA: Forms a functional complex with LbaCas12a and is specifically activated by the target sequence. (LbaCas12a crRNA scaffold sequence: 5’-UAAUUUCUACUAAGUGUAGAU-3’)
Application
The CRISPR Cas12a DNA Detection Kit (Two-Step) is used for rapid DNA detection. First, perform isothermal amplification, then the amplification products activate Cas12a, generating a fluorescent signal. This enables rapid detection of DNA pathogens.
Related:
- CRISPR Cas12a-RPA DNA Lyophilized Detection Kit (One-Step)
- CRISPR Cas12a-RPA Lyophilized DNA Detection Kit (Two-Step)
- CRISPR Cas12a-RPA DNA Lyophilized Detection Kit (Two-Step) (Test Strip)
- CRISPR Cas13a-RPA DNA Lyophilized Detection Kit (One-Step)
- CRISPR Cas13a-RPA DNA Lyophilized Detection Kit (Two-Step)
- CRISPR Cas13a-RPA DNA Lyophilized Detection Kit (Two-Step) (Test Strip)
- CRISPR Cas12a-RPA RNA Lyophilized Detection Kit (One-Step)
- CRISPR Cas12a-RPA Lyophilized RNA Detection Kit (Two-Step)
- CRISPR Cas12a-RPA RNA Lyophilized Detection Kit (Two-Step) (Test Strip)
- CRISPR Cas13a-RPA RNA Lyophilized Detection Kit (One-Step)
- CRISPR Cas13a-RPA RNA Lyophilized Detection Kit (Two-Step)
- CRISPR Cas13a-RPA RNA Lyophilized Detection Kit (Two-Step) (Test Strip)
- Lateral Flow Assay for CRISPR SHERLOCK
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