Recombinant Human GM-CSF Protein
- 91102ES08
- 翌圣
- 上海市
- 现货
- 5 μg
- 100 μg
- 500 μg
- 1mg
- 815.00 元
- 2023-04-10 13:36:22
翌圣生物科技（上海）股份有限公司
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Product Information
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Product Name
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Cat#
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Size
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Price（¥）
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Recombinant Human GM-CSF Protein
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91102ES08
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5 μg
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815.00
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91102ES60
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100 μg
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6815.00
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91102ES76
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500 μg
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20425.00
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91102ES80
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1 mg
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31625.00
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Product Description
Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) is a powerful growth and differentiation factor which acts on hematopoietic progenitor cells and also activates differentiated granulocytes and macrophages. The 22 kDa glycosylated GM CSF, similar to IL 3 and IL 5, is a cytokine with a core of four bundled alpha -helices. Mature human GM CSF shares 63% 70% amino acid sequence identity with canine, feline, porcine, and rat GM CSF and 54% with mouse GM CSF. GM-CSF has been and is still widely used as an adjuvant in clinical trials of vaccination with autologous tumor cells, peptides and/or dendritic cells in a variety of human neoplasms. The effects of GM-CSF on HIV-1 replication in primary monocytes and macrophages, and in related cell lines, have demonstrated augmentation of HIV-1 expression in vitro.
Product Properties
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Synonyms
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Granulocyte/Macrophage Colony-Stimulating Factor, GM-CSF, CSF-2, MGI-1GM, Pluripoietin-α
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Accession
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P04141
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GeneID
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1437
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Source
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E.coli-derived human GM-CSF protein, Ala18-Glu144.
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Molecular Weight
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Approximately 14.5 kDa.
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AA Sequence
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APARSPSPST QPWEHVNAIQ EARRLLNLSR DTAAEMNETV EVISEMFDLQ EPTCLQTRLE LYKQGLRGSL TKLKGPLTMM ASHYKQHCPP TPETSCATQI ITFESFKENL KDFLLVIPFD CWEPVQE
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Tag
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None
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Purity
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> 98% by SDS-PAGE and HPLC analyses.
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Biological Activity
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The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.1 ng/mL, corresponding to a specific activity of > 1.0 × 107 IU/mg. Fully biologically active when compared to standard.
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Endotoxin
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< 1.0 EU per 1μg of the protein by the LAL method.
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Formulation
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Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
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Reconstitution
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We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.
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Shipping and Storage
The products are shipped with ice pack and can be stored at -20 ℃ for 1 year.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 °C under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Cautions
1.Avoid repeated freeze-thaw cycles.
2.For your safety and health, please wear lab coats and disposable gloves for operation.
3.For research use only.
