产品描述
 
FAP (also known as seprase) is a 95 kDa Type II transmembrane serine protease that is structurally related to dipeptidyl peptidase IV (DPPIV/CD26). Within the extracellular domain (ECD), human FAP shares 90% amino acid (aa) sequence identity with mouse and rat FAP. Alternative splicing of human FAP generates a truncated isoform that consists of the C-terminal 239 residues of the ECD. A soluble and enzymatically active form of FAP known as antiplasmin-cleaving enzyme (APCE) circulates in human plasma. FAP is expressed on reactive stromal fibroblasts in tumor tissue and wound healing and on synoviocytes in rheumatoid arthritis. It exhibits dipeptidyl peptidase activity with substrate specificity similar to DPPIV, which is specific for N-terminal Xaa-Pro sequences. FAP is also an endopeptidase that can degrade Gelatin, Collagens I and IV, Fibronectin, and Laminin as well as several peptide hormones (e.g. Neuropeptide Y, Brain Natriuretic Peptide, Substance P, Peptide YY, and Incretins). The enzymatic activity is dependent on FAP association with DPPIV on the cell surface. The matrix-dedgrading activity of FAP contributes to tumor cell migration and invasion. In addition, FAP can enhance tumor cell growth by limiting the development of anti-tumor immunity.
 
产品性质
 
别名FAP; FAPalpha; SIMP; Seprase; APCE; DPPIV; DPPIVA; FAPAUniprot No.Q12884.1表达区间及表达系统Recombinant Human FAP Protein is expressed from HEK293 Cells with His tag at the N-terminal. It contains Leu26-Asp760.分子量Approximately 86.1 kDa. Due to glycosylation, the protein migrates to 90-100 kDa based on Tris-Bis PAGE result.纯度> 95% as determined by SDS-PAGE and HPLC.活性ELISA Data：Immobilized Human FAP, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-FAP Antibody, hFc Tag with the EC50 of 25.8ng/ml determined by ELISA.内毒素< 1.0 EU per 1μg of the protein by the LAL method.制剂Lyophilized from 0.22μm filtered solution in 20mM Tris, 250mM NaCl (pH 8.2). Normally 8% trehalose is added as protectant before lyophilization.复溶Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
 
运输与保存方法
 
冰袋运输。-20℃至-80℃保存，一年有效期。
复溶后，-20 至 -80°C，在未开封状态下保存 3-6 个月。复溶后，2-8°C 保存 2-7 天。
建议第一次使用时分装冻存，避免反复冻融。

 注意事项
 
1. 避免反复冻融。
2. 为了您的安全和健康，请穿实验服并戴一次性手套操作。
3. 本产品仅作科研用途！
 
产品数据