血清替代物 CDM-HD Serum Replacement

Optimized for Hollow Fiber Bioreactors

• Increased buffering capacity maintains optimum conditions for protein and antibody production.

• No detergents or other cell membrane stabilizing agents that sometimes interfere with assays or precipitations.

• Composition balanced for high density culture.

Notes:

• CDM-HD will not require any adaptation but is optimized for cells growing at high density. For best results switch to CDM-HD after cells have reached a high density inside the FiberCell^® Systems hollow fiber bioreactor, typically after one week of culture or so.

• CDM-HD does not contain any carbon source. When working with a low glucose medium such as RPMI it is important to supplement the glucose concentration to 4.5 grams per liter.

• CDM-HD is protein free and does not contain any cell attachment factors. When innoculating cells in medium containing CDM-HD add 10% FBS to the cell innoculum only. This will provide the required attachment factors for adherent cells. This is not required for suspension cells though hybridoma cell lines should be treated as adherent cells.

• CDM-HD can be used in other culture systems such as spinner culture and roller culture. For these applications add plurionic F60 or some other cell membrane protecting surfactant.

• CDM-HD is protein free. The secreted protein of interest may be the only significant protein present in your cell culture supernatant. You should re-evaluate your purification protocols as entire steps can sometimes be eliminated, increasing yield. Keep in mind that CDM-HD is protein free and contains no ferritin so the free iron levels will be higher than in standard mediums. Pay attention to any chelating agents that may be part of your buffers or purification protocol.