产品介绍

• 宿主来源

  Rabbit

• 分子别名

  Sarcoma antigen 1, Cancer/testis antigen 14 (CT14), SAGE

• 细胞定位

  Nucleus

• Accession

  
  Q9NXZ1

• 克隆号

  SDT-317-60

• 抗体类型

  Recombinant mAb

• 应用

  IHC-P

• 反应种属

  Hu

• 纯化方式

  Protein A

• 浓度

  0.5 mg/ml

• 标记

  Unconjugated

• 性状

  Liquid

• 缓冲体系

  PBS

• 储存条件

  12 months from date of receipt / reconstitution, 4 °C as supplied

• • IHC

    1:500

• Cancer/testis (CT) antigens including MAGEA, NY-ESO-1, GAGE, CT7, CT10, CT45, SAGE1, and NXF2 are protein antigens that are normally expressed in the germ cells of adult testis and developing fetal testis and ovary, but not in any other adult tissues. Examination of various types of human cancer showed CT gene activation and protein expression in a proportion of human cancers in a lineage-unrelated fashion. Due to this restricted pattern of expression, CT antigens are often recognized by the immune system of cancer patients, and this spontaneous immunogenicity raises the possibility of their use as therapeutic cancer vaccine targets.

• 免疫组化

  • 

    IHC shows positive staining in paraffin-embedded human testis. Anti- SAGE1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 

    Negative control: IHC shows negative staining in paraffin-embedded human colon. Anti- SAGE1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 

    Negative control: IHC shows negative staining in paraffin-embedded human tonsil. Anti- SAGE1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 

    IHC shows positive staining in paraffin-embedded human lung cancer. Anti-SAGE1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 

    IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti- SAGE1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

  • 

    IHC shows positive staining in paraffin-embedded human lung cancer. Anti- SAGE1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.