磷酸化IRE1蛋白抗体
phospho-IRE1 (Ser724) Rabbit pAb
- bs-16698R
- 北京博奥森
- 北京市
- 现货
- 50ul
- 100ul
- 200ul
- 议价
- 2023-10-12 16:57:01
北京博奥森生物技术有限公司
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- 英文名称
- phospho-IRE1 (Ser724) Rabbit pAb
概述
产品编号
bs-16698R
产品分类
一抗
产品类型
质检1级
英文名称
phospho-IRE1 (Ser724) Rabbit pAb
中文名称
磷酸化IRE1蛋白抗体
英文别名
IRE1 (phospho S724); p-IRE1 (phospho S724); Endoplasmic reticulum (ER) to nucleus signalling 1; Endoplasmic reticulum to nucleus signaling 1; Endoplasmic reticulum-to-nucleus signaling 1; Endoribonuclease; ER to nucleus signaling 1; ER to nucleus signalling 1; ERN 1; ERN1; ERN1_HUMAN; hIRE 1p; hIRE1p; Inositol requiring 1; Inositol requiring 1, S. cerevisiae, homolog of; Inositol requiring enzyme 1, S. cerevisiae, homolog of; Inositol requiring protein 1; inositol-requiring enzyme 1; Inositol-requiring protein 1; IRE 1; IRE 1a; IRE 1P; Ire1 alpha; Ire1-alpha; IRE1a; Ire1alpha; IRE1P; MGC163277; MGC163279; Protein kinase/endoribonuclease; RGD1559716; Serine/threonine protein kinase/endoribonuclease IRE1.
交叉反应
Rat,Mouse,Human（predicted：Sheep,Horse,Cow,Pig）
抗体来源
Rabbit
免疫原
KLH conjugated synthesised phosphopeptide derived from human IRE1 around the phosphorylation site of Ser724
亚型
IgG
纯化方法
affinity purified by Protein A
克隆类型
Polyclonal
理论分子量
107kDa
浓度
1mg/ml
储存液
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Subunit
Homodimer; disulfide-linked. Dimer formation is driven by hydrophobic interactions within the N-terminal luminal domains and stabilized by disulfide bridges. Also binds HSPA5, a negative regulator of the unfolded protein response. This interaction may disrupt homodimerization and prevent activation of ERN1. Interacts with TAOK3 and TRAF2.
Subcellular Location
Endoplasmic reticulum membrane; Single-pass type I membrane protein.
Tissue Specificity
Ubiquitously expressed. High levels observed in pancreatic tissue.
Post-translational modifications
Autophosphorylated.
Similarity
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 1 KEN domain.
Contains 1 protein kinase domain.
Contains 1 KEN domain.
Contains 1 protein kinase domain.
Database links
Entrez Gene: 10595 Human
Entrez Gene: 26918 Mouse
Omim: 604034 Human
SwissProt: O75460 Human
SwissProt: Q9EQY0 Mouse
Unigene: 133982 Human
Unigene: 20452 Mouse
背景资料
ERN2, endoplasmic reticulum to nucleus signaling 2, induces translational repression through 28S ribosomal RNA cleavage in response to ER stress. It is pro-apoptotic and appears to play no role in the unfolded-protein response, unlike closely related proteins.
应用
| 应用 | 推荐稀释比例 |
|---|---|
| IF | 1:100-500 |
| IHC-F | 1:100-500 |
| WB | 1:500-2000 |
图片资料
Paraformaldehyde-fixed, paraffin embedded (rat pancreas tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IRE1 (Ser724)) Polyclonal Antibody, Unconjugated (bs-16698R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IRE1 (Ser724)) Polyclonal Antibody, Unconjugated (bs-16698R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IRE1 (Ser724)) Polyclonal Antibody, Unconjugated (bs-16698R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Sample:
Lane 1: Human 293T cell lysates
Lane 2: Human DU145 cell lysates
Lane 3: Human HeLa cell lysates
Lane 4: Human Jurkat cell lysates
Primary: Anti-phospho-IRE1 (Ser724) (bs-16698R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 107 kDa
Observed band size: 130 kDa
Lane 1: Human 293T cell lysates
Lane 2: Human DU145 cell lysates
Lane 3: Human HeLa cell lysates
Lane 4: Human Jurkat cell lysates
Primary: Anti-phospho-IRE1 (Ser724) (bs-16698R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 107 kDa
Observed band size: 130 kDa
