Cat. No.LV053
Name3rd Generation Packaging System MixUnit200µlDescription

For the production of lentiviral particles, three components are generally required: 1) a lentiviral vector containing your inserts of interest, 2) one or two packaging vectors which contain all necessary viral structure proteins, 3) an envelope vector expressing Vesicular Stomatitis Virus (VSV) glycoprotein (G).

The 3rd generation packaging system offers maximal biosafety as the lentiviral Rev gene is supplied as an independent vector from other structure genes, further eliminating the possibility of reverse recombination of vectors into replication competent viral particles.

The 3rd generation lentiviral packaging mix will only support lentiviral expression vectors with a chimeric 5' LTR in which the HIV promoter is replaced with CMV or RSV, thus making it TAT-independent. The 3rd generation lentiviral vectors will not support the production of 2nd generation lentiviral particle productions. All of the lentiviral vectors marketed by ABM are TAT-independent.

Components (200ul mix of 3 plasmids):

pLenti-P3A

pLenti-P3B

pLenti-P3C

• Selection: Ampicillin (Bacterial Selection)
• Amount: 200 μl (100 µg mixture of pLenti-P3A, pLenti-P3B, and pLenti-P3C)
• Appearance: Liquid
• Storage: Temperature °20-C or below (shipped at ambient temperature)
• Shelf Life: Up to 1 year (when at °20-C or below in a frost-free freezer)

*User Manual Refer to website for downloadable user manual

Additional Information

Shelf Life: Up to 1 year (when stored at -20°C in a frost-free freezer).

Note

NOT FOR RESALE without prior written consent of ABM. This product is distributed for laboratory research only.

Material CitationIf use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. LV053