Anti ALK Rabbit Polyclonal Antibody
- CR-R21280
- 科博瑞生物
- 湖北省武汉市
- 现货
- 100ul
- 2000 元
- 2024-06-10 17:53:26
科博瑞（武汉）生物科技有限公司
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- 种属
- Mouse
ALK [CR-R21280]
[100 µL]
| Catalog Number: CR-R21280 |
| Gene Symbol: ALK, CD246, NBLST3 |
|
Description: Anti ALK Rabbit Polyclonal Antibody
|
| Background: Anaplastic lymphoma kinase (ALK) also known as ALK tyrosine kinase receptor or CD246 (cluster of differentiation 246) is a neuronal orphan receptor tyrosine kinase. ALK is essentially and transiently expressed in specific regions of the central and peripheral nervous systems and plays an important role in the genesis and differentiation of the nervous system. Abnormal ALK, by fusion with other genes, gaining additional gene copies or mutations, can be oncogenic. |
| Immunogen: Purified ALK protein, human origin. |
| Applications: ELISA, WB, IHC |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:100-1:1000 IHC: 1:50-1:200 |
| Concentration: 0.2mg/ml |
| Host: Rabbit |
| Format: Liquid |
| Clonality: Polyclonal |
| Isotype: |
| Purity: Purified from serum |
| Storage buffer: |
| Preservative: no |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes ALK of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
| Western blot: |
 |
Western blot analysis of recombinant ALK protein. Purified His-tagged ALK protein was blotted with Anti ALK rabbit polyclonal antibody (Cat. #CR-R21280). |
| Immunohistochemistry: |
 |
Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue with Anti ALK polyclonal antibody (Cat. #CR-M21280). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50 dilution) overnight at 4°C. A HRP-conjugated Goat anti-Rabbit IgG (1:50 dilution) was used as secondary antibody. |
