辣根过氧化物酶标记亲和纯化羊抗兔IgG (H+L)(进口分装)
- bs-40295G-HRP
- 北京博奥森
- 北京市
- 现货
- 100ul
- 1ml
- 议价
- 2023-10-11 12:00:31
北京博奥森生物技术有限公司
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概述
产品编号
bs-40295G-HRP
产品分类
标记二抗
英文名称
AffiniPure Goat Anti-Rabbit IgG H&L / HRP
中文名称
辣根过氧化物酶标记亲和纯化羊抗兔IgG (H+L)(进口分装)
英文别名
AffiniPure Goat Anti-Rabbit IgG (H+L) (min X Hu, Ms, Rat Sr Prot)/HRP; <br> AffiniPure Goat Anti-Rabbit IgG H&L/HRP; <br> AffiniPure Goat Anti-Rabbit IgG H&L(HRP); <br> HRP AffiniPure Goat Anti-Rabbit IgG (H+L); <br> Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L)
中文别名
HRP标记山羊抗兔IgG (H+L); <br> 辣根过氧化物酶标记羊抗兔IgG (H+L)(进口分装);<br>
交叉反应
Rabbit
标记
HRP
抗体来源
Goat
免疫原
Native Rabbit IgG
亚型
IgG
纯化方法
affinity purified by Protein G
克隆类型
Polyclonal
浓度
1.0 mg/ml
储存液
10 mM TBS (pH=7.4) with 1% BSA, 0.03% Proclin300 and 50% glycerol.
保存条件
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Subunit
背景资料
HRP Conjugated Goat (polyclonal) Anti-Rabbit IgG(H+L), Highly Cross Adsorbed
HRP antibodies can be used for a variety of applications, including ELISA, Western blotting, In-Cell Western assays, In-Gel Westerns, and many others.
Isolation of specific antibodise was accomplished by immunoaffinity chromatography using antigens immobilized on agarose beads. Based on immunoelectrophoresis, this antibody reacts with the heavy chains of rabbit IgG as well as the light chains common to most rabbit immunoglobulins. No reactivity was detected against non-immunoglobulin serum proteins. This antibody was tested by ELISA and/or solid phase adsorbed for minimal cross-reactivity with human, mouse, and rat serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot and ELISA applications.
HRP antibodies can be used for a variety of applications, including ELISA, Western blotting, In-Cell Western assays, In-Gel Westerns, and many others.
Isolation of specific antibodise was accomplished by immunoaffinity chromatography using antigens immobilized on agarose beads. Based on immunoelectrophoresis, this antibody reacts with the heavy chains of rabbit IgG as well as the light chains common to most rabbit immunoglobulins. No reactivity was detected against non-immunoglobulin serum proteins. This antibody was tested by ELISA and/or solid phase adsorbed for minimal cross-reactivity with human, mouse, and rat serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot and ELISA applications.
应用
| 应用 | 推荐稀释比例 |
|---|---|
| ELISA | 1:5000-20000 |
| IHC-F | 1:3000-5000 |
| IHC-P | 1:3000-5000 |
| WB | 1:5000-20000 |
图片资料
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (B MyB) Polyclonal Antibody, Unconjugated (bs-5960R) at 1:200 overnight at 4°C, Secondary antibody was Goat Anti-Rabbit IgG H&L (HRP) (bs-40295G-HRP ) at 1/200 dilution and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (B MyB) Polyclonal Antibody, Unconjugated (bs-5960R) at 1:200 overnight at 4°C, Secondary antibody was Goat Anti-Rabbit IgG H&L (HRP) (bs-40295G-HRP ) at 1/200 dilution and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (B MyB) Polyclonal Antibody, Unconjugated (bs-5960R) at 1:200 overnight at 4°C, Secondary antibody was Goat Anti-Rabbit IgG H&L (HRP) (bs-40295G-HRP ) at 1/200 dilution and DAB staining.
