Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Granzyme B Polyclonal Antibody, Unconjugated(bs-1351R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
颗粒酶B抗体
Granzyme B Rabbit pAb
- bs-1351R
- 北京博奥森
- 北京市
- 现货
- 50ul
- 100ul
- 200ul
- 议价
- 2023-10-18 16:12:14
北京博奥森生物技术有限公司
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- 英文名称
- Granzyme B Rabbit pAb
概述
产品编号
bs-1351R
产品分类
一抗
产品类型
质检1级
英文名称
Granzyme B Rabbit pAb
中文名称
颗粒酶B抗体
英文别名
GranzymeB; Granzyme-B; C11; Cathepsin G-like 1; CTLA-1; Cathespin G Like 1; CCPI; CCP-1; CGL 1; CGL1; CSP B; CSPB; CTLA 1;CTLA1; CTSGL 1; CTSGL1; Cytotoxic serine protease B; Cytotoxic T Lymphocyte Associated Serine Esterase 1; Cytotoxic T lymphocyte proteinase 2; Fragmentin 2; Fragmentin2; Granzyme 2; Granzyme2; GRB; GZMB; Cytotoxic T-lymphocyte proteinase 2; Fragmentin-2; GRAB_HUMAN; Granzyme B (granzyme 2, cytotoxic T lymphocyte associated serine esterase 1); Granzyme B; Granzyme-2; GranzymeB; HLP; T cell serine protease 1 3E; T cell serine protease 1-3E; T-cell serine protease 1-3E; HLP; Human lymphocyte protein; Lymphocyte protease; Protease Serine B; SECT; AA 18-29; T cell serine protease 1.
交叉反应
Human（predicted：Mouse,Rat,Pig,Cow）
抗体来源
Rabbit
免疫原
KLH conjugated synthetic peptide derived from human GZMB
亚型
IgG
纯化方法
affinity purified by Protein A
克隆类型
Polyclonal
理论分子量
25kDa
浓度
1mg/ml
储存液
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
功能
This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.
亚基
亚细胞定位
Cytoplasmic granule. Note=Cytoplasmic granules of cytolytic T-lymphocytes and natural killer cells.
相似性
Belongs to the peptidase S1 family. Granzyme subfamily.
Contains 1 peptidase S1 domain.
Contains 1 peptidase S1 domain.
数据库链接
Entrez Gene: 3002 Human
Entrez Gene: 14939 Mouse
Omim: 123910 Human
SwissProt: P10144 Human
SwissProt: P04187 Mouse
Unigene: 1051 Human
Unigene: 14874 Mouse
Unigene: 132115 Rat
Unigene: 145789 Rat
Unigene: 21395 Rat
背景资料
Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein encoded by this gene is crucial for the rapid induction of target cell apoptosis by CTL in cell-mediated immune response. [provided by RefSeq, Jul 2008]
应用
| 应用 | 推荐稀释比例 |
|---|---|
| ELISA | 1:5000-10000 |
| Flow-Cyt | 2ug/Test |
| IF | 1:100-500 |
| IHC-F | 1:100-500 |
| IHC-P | 1:100-500 |
图片资料
Blank control:U937.
Primary Antibody (green line): Rabbit Anti-Granzyme B antibody (bs-1351R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at PBST. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Primary Antibody (green line): Rabbit Anti-Granzyme B antibody (bs-1351R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at PBST. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
