重组人 HGF蛋白（Recombinant Human HGF Protein），GMP级

 

产品货号：GMP-TL765

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产品描述（Description）

 

HGF is a hetrodimer molecule composed of 69-kDa alpha-subunit and 34-beta-subunit. HGF is a potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization. HGF mRNA and HGF activity increase markedly in the liver of rats after various liver injuries such as hepatitis, ischemia, physical crush, and partial hepatectomy. Production of HGF in the liver occurs in Kupffer cells and sinusoidal endothelial cells, but not in parenchymal hepatocytes. HGF mRNA is also markedly increased even in the intact lung, kidney, and spleen after injuries of the liver. Therefore, HGF may act as a trigger for liver regeneration through two mechanisms: a paracrine mechanism and an endocrine mechanism. Moreover, HGF mRNA increases markedly in the kidney after various renal injuries, thus it suggests that HGF may act not only as a hepatotropic factor but also as a renotropic factor. HGF stimulates growth of various epithelial cells, including renal tubular cells (Mitogen). It is worth noting that HGF strongly enhances motility of epithelial cells (Motogen) and induces epithelial tubule formation (Morphogen), while it strongly inhibits growth of several tumor cells. All these findings indicate that HGF may have important roles in organogenesis, morphogenesis, carcinogenesis, as well as in organ regeneration.

 

 

 

表达宿主（Expression host） 

  

HEK293细胞

 

 

 

同义词（Synonyms）

 

Hepatocyte growth factor ;DFNB39 Protein, Human; F-TCF Protein, Human; F-TCFB Protein, Human; HGFB Protein, Human; HPTA Protein, Human; SF Protein, Human.

 

 

 

蛋白序列（Protein Sequence）

 

DNA序列编码人HGF（P14210）表达带有His标签在C末端

 

 

 

分子量（Molecular Weight）

 

重组人HGF蛋白包含742个氨基酸，预测分子量为84.8kD

 

 

 

纯度（Purity）

 

>90%，采用 SDS-PAGE 凝胶分析

 

 

 

内毒素（Endotoxin）

 

<0.1EU/μg

 

 

 

生物活性（Biological Activity）

 

 

 

 

 

纯化方式（Purification Method）

 

层析纯化

 

 

 

性状（Character）

 

白色疏松体

 

 

 

使用说明（Instructions）

 

 

冻干制剂可在 2~8℃保存 24 个月。 

如需分装，可用注射用水、生理盐水、培养基或 PBS 溶解，溶解后分装成小份，置于-20℃保存 期 6 个月，-80℃保存期 12 个月，稀释后浓度不低于 100μg/mL。避免反复冻融。

 

 

   注意：For research and manufacturing use.