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  Anti Active Gαo Mouse Monoclonal Antibody
  • CR-M26907
  • 科博瑞生物
  • 湖北省武汉市
  • 现货
  • 30ul
  • 4800
  • 2024-06-12 15:30:52

科博瑞(武汉)生物科技有限公司

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  • 种属
  • Mouse

Active Gαo GTP [CR-M26907]
[30 µL]

Catalog Number: CR-M 26907
Gene Symbol:  Gnao

Description:   Anti Active Gαo Mouse Monoclonal Antibody

 

Background:   Heterotrimeric G proteins are critical cellular signal transducers. Gαorepresents one sub-family of G proteins that could mediate the inhibition of adenylyl cyclases. Other biochemical and physiological functions of Gαo proteins are being explored.
Immunogen:   Recombinant full length protein of active Gαo
Applications:   IP, IHC
Recommended Dilutions:   1 µg for 1~2 mg total cellular proteins for IP; 
                                                   1:100 dilution for IHC
Concentration:   1 mg/ml
Host:   mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG1
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Anti-active Gαo antibody recognizes active Gαo of vertebrates.
Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles
 
Immunoprecipitation/Western blot:  
o activation assay. Purified Gαo proteins were loaded as a control (lanes 1) or immunoprecipitated after treated with GDP (lane 2) or GTPγS (lane 3). Immunoprecipitation was done with the anti active Gαo monoclonal antibody (Cat. No. CR-M26907). Immunoblot was with an anti Gαopolyclonal antibody (Cat. No. CR-M21015).

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