His tag标签抗体
His tag
- bs-0287R
- 北京博奥森
- 北京市
- 现货
- 按需
- 议价
- 2023-10-18 09:41:19
北京博奥森生物技术有限公司
一键申请试用
咨询
加入意向单
联系方式
- 英文名称
- His tag
- 别名
- HisX6; 6His; His-Tag; 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; Histidine.
| 产品类型 | 标签抗体 |
| 研究领域 | 肿瘤 细胞生物 免疫学 染色质和核信号 信号转导 合成与降解 结合蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Recombinant protein |
| 产品应用 | WB=1:1000-5000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Synthetic peptide: HHHHHH conjugated to KLH |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 | The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. SWISS: N/A Gene ID: N/A 标签抗体 蛋白质纯化的新途径─应用His-Tag抗体检测His-Tag融合蛋白 1） 6xHis与不同的Sepharose纯化填料结合（捆绑），可不受构象的影响，在天然或变形的条件下可进行进一步纯化。 |
| 产品图片 |
 Sample:
Lane1: His-Tagged Fusion Protein Overexpression E.coli Lysate at 2ug Lane2: His-Tagged Fusion Protein Overexpression E.coli Lysate at 2ug Primary: Anti-His tag (bs-0287R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41 kD Observed band size: 45 kD  Sample:
Lane 1: His-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41403P) at 2ug Lane 2: His-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-41403P) at 4ug Primary: Anti-His tag (bs-0287R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51 kD Observed band size: 51 kD |
