This antibody was raised from rabbits that were immunized with N^G-mono-methyl arginine-KLH conjugates and was specifically isolated and affinity purified using the monomethyl-arginine antigen. The affinity purified methyl arginine antibodies are conjugated to peroxidase (HRP) and are useful for detection, isolation and localization of proteins with N^G-methylated arginine residues.

 

Rabbit

250 µg/mL in stabilizing buffer with 50% glycerol

N⁵-mono-methyl arginine-KLH conjugates

Antigen-specific affinity chromatography followed by absorption with dimethyl arginine

Reductive amination; HRP/Ab ratio: 2:1

The antibody recognizes proteins with methylated arginine residues. It does not cross react with acetylated proteins or mono- and dimethylated proteins.

Human, mouse and pig

ELISA (1:2000); WB (1:1000); IP (5 µg/mg protein sample); IF (5 µg/mL)

Arginine methylation is a very common post-translational modification that occurs predominantly in the nucleus. The main pool of proteins that are modified possess RNA binding properties. More broadly, arginine methylation has been implicated in a variety of cellular processes including protein trafficking, signal transduction and transcriptional regulation.  

Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is six months from date of shipping if stored properly.