Trimethyl Lysine Antibody,HRP
Trimethyl Lysine Antibody,HRP
- ICP0602
- ImmuneChem
- 一周内
- 100ug支
- 3880 元
- 2022-08-03 17:21:29
南宁市蓝光生物技术有限公司
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- 英文名称
- Trimethyl Lysine Antibody,HRP
详细介绍
Tri-methyl Lysine Antibody (pan specific), HRP
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Catalog# Product Description |
ICP0602 This rabbit polyclonal antibody is isolated and affinity purified from the anti-serum against chemically methylated protein antigen. The affinity purified trimethylated lysine antibodies are affinity absorbed with N-(epsilon) mono- and di-methylated lysine. The purified antibody is conjugated to horse radish peroxidase (HRP) via reductive amination. Direct labelling of primary antibodies will avoid the use of secondary antibodies therefore eliminating the effects of the secondary antibody-conjugates. The antibodies could be utilized for detection and quantization of proteins with N-trimethylated lysine residues. |
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 Western blot analysis of trimethylated protein from human melanoma cells (MMRU) with ICP0602
A: Competitive inhibition with N-(epsilon) Trimethyl-lysine (50µg/mL)
B: Without competitive inhibition with N-(epsilon) trimethyl lysine
 Western blot analysis of trimethylated protein from the acid extract of calf spleen with ICP0602
C: Competitive inhibition with N-(epsilon) trimethyl lysine (50µg/mL)
D: Without competitive inhibition with N-(epsilon) trimethyl lysine
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Formulation
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500 µg/mL in HRP stabilizing buffer with 50% glycerol
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Immunogen
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Methylated KLH conjugates
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Purification
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N-(epsilon) trimethyllysine on agarose and immunoaffinity absorbed with N-(epsilon) methylated lysine and N-(epsilon) dimethylated lysine on agarose
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Conjugation
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Reductive amination; HRP/Ab molar ratio - 2:1
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Specificity
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Recognizes proteins with trimethylation on lysine residues (N-epsilon); antibody does not cross react with acetylated proteins or mono- and dimethylated proteins.
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Species Tested
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Human, rat, mouse, bovine and pig
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Applications
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ELISA (1:5000); WB (1:1000)
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Scientific Description
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In the biosynthetic pathway of 1-carnitine, epsilon-N-trimethyllysine hydroxylase is the first enzyme that catalyzes the formation of beta-hydroxy-N-epsilon-trimethyllysine from epsilon-N-trimethyllysine. This reaction is dependent on alpha ketoglutarate, Fe2+, and oxygen.
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Storage & Stability
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Product is stable for several weeks at 4°C. For extended storage, and store product at -20°C. Expiration date is six months from date of shipping if stored properly.
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Product Specific References
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1. J. Biol. Chem. 2001. 36(276): 33512-33517. doi:10.1074/jbc.M105929200
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